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    Team Leader Transportation and Logistics - Riyadh, المملكة العربية السعودية - Almarai saudi

    Almarai saudi
    Almarai saudi Riyadh, المملكة العربية السعودية

    منذ أسبوع

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    وصف

    A fully funded 4-year position is availablein the laboratory Structure and Function ofMembrane Proteins at the VIB-VUB Centerfor Structural Biology, Brussels, The project focuses on thestructural elucidation of membrane-remodeling protein complexesfrom the endoplasmic reticulum and integrates fundamental cellbiology and structural biology by cryo-electron

    The asymmetrical distribution of lipids in biologicalmembranes is a well-established biological paradigm and essentialfor signaling and organelle-/protein While the regulated asymmetryin the plasma membrane of eukaryotic cells is well-studied, muchless is known about the symmetry or asymmetry of lipid bilayers inintracellular The endoplasmic reticulum plays a central role inthis context as lipids are retrieved from its cytosolic leaflet forexport to acceptor Interleaflet balance and availability of lipidspecies in the leaflets of the endoplasmic reticulum membrane aregoverned by scramblases, a functional lipid transporter class(Brunner et , Nature, 2014) that only recently was recognized to berequired for non-vesicular lipid transport and as essential hostfactor(s) for virus

    We aim to structurallycharacterize scramblases involved in lipid export from theendoplasmic reticulum and reveal their roles, organization, andinteractome in the In addition, the mechanisms of viral hijackingof scramblase host factors for the assembly and spread of virusesare a matter of

    We apply state-of-the-artmethods for the expression and purification of delicate membraneprotein complexes in cultured mammalian cells and their subsequentbiophysical and biochemical characterization by HPLC, SEC-MALS, andfunctional reconstitution into Proteins will be furthercharacterized in living cells by confocal microscopy located in theFor structural work, single particle cryo-electron microscopy isextensively utilized at Belgium s prime location, the BiologicalElectron Cryogenic Microscopy Center of Flanders (BECM) on the VUB

    Profile

    Candidates musthold a Master s in biochemistry, biophysics, or related A keeninterest in eukaryotic cell biology, membrane proteins, andstructural biology is essenti The ability to work organized andindependently with excellent communication skills is mandatory aswell as proficiency in written and spoken Previous experience instructural biology (cryo-EM), membrane protein purification, orcell biology will be Candidates should demonstrate a strongmotivation and commitment to solving scientific problems and acuriosity-driven approach in their

    Weoffer

    • A fully funded position for 4Starting date upon
    • Excellent opportunity for achallenging membrane protein structure project with translationalaspects embedded in the core expertise of theteam
    • Extensive and excellent training forcryo-EM (sample preparation, data collection, andprocessing)
    • Dedicated new cell culture lab foradherent or suspension culture of mammaliancells
    • State-of-the-art systems for purificationand analysis in vitro and in living cells
    • Theapplicant will benefit from access to centralized facilities (inparticular Nanobody technology, proteomics, cutting-edgemicroscopy, and structural biology).

    How to apply?

    Please apply onlinewith a cover letter detailing your background and interest in thisposition and your full CV including the contact information of tworeferees (with email, address, and phone number).

    For further information visit and contact Janine Brunner().

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